Go up to your room, and the next thing you you expect is a clean room, airy, no musty stale smell. Bed should look comfortable. A reasonable sized television with at least some international channels (especially if you are in a country that had a very different language). A work desk or table for those evenings when you need to catch up on work. I actually had the misfortune in Bangalore to have been assigned a room which turned out to be occupied. Luckily the tenant was not in his room when I opened it. I saw clothes strewn over the bed, shoes and luggage opened in the cupboard. That should never happen!
Then you have a quick check of the bathroom/toilet. Are there toiletries provided? The only ones I I really need from the hotel are normally the shampoo. I bring my own toothbrush, toothpaste, comb, razor, etc. Some of the classier hotels provides branded toiletries. Sometimes I bring them home for use during personal holidays.
For this update, I want to mention about something a little different that I got from Crowne Plaza in Gurgaon. I have only seen similar offerings from one other hotel in Beijing.
Crowne Plaza has a pillows menu!! 
Almost every hotel has room service but a pillow menu is a refreshing difference. They had a "wheat pillow", an "anti-stress" pillow, a "contour" pillow, "synthetic" pillows of varying softness and so on. I was pretty satisfied with what was provided when I checked in but I am sure that many people would appreciate the pillow menu.
Crown Plaza also provided a little blurb about keeping fit nutritionally. That's always helpful for a regular traveller (like yours truly).
Last but not least, the hotel also offered spa and massages, even in-room. My only gripe is that the prices are way too high.
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